In human melanoma lines and a mouse model, the neuronal morphogenesis E3 ligase TRIM9 shifts cells between bleb-based and adherent states via VASP ubiquitylation — though key in vivo findings are underpowered and the mechanistic chain is largely correlational.

FRET-plus-patch-clamp experiments in four human cancer-derived lines show mild depolarization activates ERK through phosphatidylserine–Ras, but evidence outside cancer lines and single-cell sample sizes (n=15–23) leave physiological relevance untested.

An unreviewed preprint shows Giantin sequesters RAD51C at the Golgi until ATM-dependent signaling releases it for DNA repair — supported by triplicate assays in three cancer-derived lines, but without genetic knock-in validation or replication.

Auxin-inducible degradation shows CENP-E transports PCM1 away from centrosomes in G2 to license Plk1-dependent maturation, though the microcephaly link is inferred and evidence rests on one patient cell-line pair.

Nasmyth's 2002 review proposed the cohesin ring/topological entrapment model later confirmed structurally, but several claims rested on unpublished data and personal communications.

Live imaging and rescue experiments in two cancer lines suggest ectopic spindle poles after haspin depletion arise indirectly from cohesion loss, not centrosome failure — a caution for interpreting multipolar phenotypes in cancer screens.

Proteomics, enzymology, and rescue experiments identify USP32 as Rab7's deubiquitinase controlling dynein transport versus retromer recycling, but SILAC arms were run with n=1 and findings lack any in vivo validation.

Biochemical and Drosophila genetic evidence shows Mical oxidation of actin strengthens cofilin severing by over ten-fold, replicated in two labs, but no pre-specified sample sizes and the structural mechanism remains inferred.

Tubulin-injection experiments in one immortalized fibroblast line show acetylation and detyrosination track enzyme localization rather than microtubule age, undermining routine PTM staining as a stability readout — but no fit statistics were reported and only two PTMs were tested.

TIRF, co-immunoprecipitation, and rescue experiments show the polarity scaffold Dlg1 recruits dynein via GKAP for cortical anchoring during astrocyte migration, though force estimates were indirect and findings confined to 2D wound assays.

A 2001 confocal study found integral tight-junction proteins but not their ZO scaffolds in the midbody of one immortalized hepatocyte line under Taxol arrest — descriptive, unquantified, and limited to a single artificial cell system.

Gallo, Vannier & Galli's 2016 review integrates contact-site tethers and PI4P-driven lipid countertransport into a field roadmap, though its tunnel-model and hemifusion claims remain speculative.

Scott & Gruenberg's 2010 review argues luminal ion composition matters beyond acidification, backed by uncoupled ClC-5/ClC-7 knock-in mice, but its later sections are explicitly speculative.

Endogenous tagging and structure-guided interface editing in C. elegans confirm RAL-1 regulates the Exocyst — strong but unreviewed preprint work whose vertebrate applicability is untested.

A 2021 review maps super-resolution and BioID approaches to centrosome biology, but 18-hour labeling windows make reported interactomes time-averaged rather than dynamic circuit diagrams.

HSP70A is confirmed as a genuine Chlamydomonas flagellar protein with >90% colocalization to IFT motor FLA10, but the cargo hypothesis rests on colocalization alone with no functional perturbation of HSP70A.

The proposed temperature-shift assay is judged feasible and cheaply decisive, but only if heat-shock-induced new synthesis is controlled — a confound convertible into a feature by pre-blocking translation.

A yeast preprint shows mitochondria carry a large steady-state ubiquitin pool cleared by the DUB Ubp3 during mitophagy, but quantification is western-blot-only with no substrate identification and incompletely stated statistics.

A 2020 review frames the paradox that actin-regulating mutants block autophagosome formation while wholesale actin depolymerization spares bulk autophagy — resolved by argument rather than experiment and hindered by reliance on unvalidated targeting predictions.

A 2025 narrative review consolidates ~130 papers positioning ATG9A as a lipid scramblase enabling phagophore expansion, but lacks quantitative synthesis and critical appraisal of observational disease studies.

Nair & Klionsky's 2005 synthesis maps early Atg-pathway logic with unusual honesty about unknowns, though it relies heavily on self-citation and offers no primary data.

A 2014 Molecular Cell preview describes PML as a SUMO-E3 directing misfolded nuclear proteins to RNF4-dependent degradation, with genetic support from an SCA1 mouse model but no direct structural evidence of the chain-length decision gate.

Senft & Ronai's 2015 review casts E3 ubiquitin ligases as regulatory nodes coordinating ER stress outcomes, a conceptually broad framework whose key links are asserted as plausible rather than mechanistically resolved.

Careful genetics implicate Chk1 in a virulence-driven G2 arrest in one fungus, but the link is correlational — based on ectopic induction in axenic culture rather than infection conditions.

h-index 20 with ~2,200 citations reflects solid quantitative-imaging work from endocytosis fundamentals to FLOT2-enabled antibody-drug conjugate sensitization, capped by small in vivo cohorts (n=3–4) and missing rescue experiments in places.

h-index 9 with two highly cited raft-localization papers, whose core conclusions were later qualified by the field's reassessment of detergent-resistant raft assays — citation counts measured influence, not mechanistic durability.

Full-text analysis finds rigor in the LDOC1 and CIN85 studies hinges on internal controls like osimertinib-unchanged comparisons, but reliance on one or two EGFR-mutant lines and no in vivo validation limits confidence.