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    Create Graphs: ORF7 deletion impact on GFP transmission (microfluidic legend)
    I can’t plot the requested ORF7-deletion β€œimpact” vs β€œGFP transmission” values because the actual microfluidic legend data (the numeric GFP transmission values per ORF7 deletion condition, plus the associated labels/units) was not provided in your message, and I’m not allowed to invent or simulate raw experimental data.
    What I need from the microfluidic legend (paste or upload)
    • All condition labels (e.g., β€œWT”, β€œΞ”ORF7”, any additional ORF7 variants, replicates).
    • GFP transmission values for each condition from the legend (mean and/or individual points; specify if values are normalized).
    • Units / definition of β€œGFP transmission” (e.g., intensity ratio, fluorescence fraction, gate-based transmission, log-scaled, etc.).
    • Whether you want an effect size computed as Ξ”ORF7 βˆ’ WT or as a ratio (and what baseline the paper uses, if any).
    • If available: error bars (SD/SEM/CI) and n.
    Once you paste the legend, I will generate
    1. Scatter + line plot: GFP transmission vs condition (with replicates if provided).
    2. Effect plot: ORF7 impact relative to baseline (difference and/or fold-changeβ€”matching your definition).
    3. Uncertainty-aware view: error bars if SD/SEM/CI are present.
    4. Raw-data table (exact numbers you provide) for auditability.
    Paste format that works best (copy/paste)
    Condition, GFP_transmission_value, (optional) SD_SEM_CI WT, ..., ... Ξ”ORF7, ..., ... [repeat for each legend entry]
    This will iteratively locate the microfluidic legend values from the relevant full-text source(s), extract the exact numeric GFP transmission entries, and then produce the Plotly graphs.


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    Updated: April 29, 2026

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