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Traceable Claims in Genomics

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     Quick Explanation


    H1047R ddPCR mutant fraction + exon context (from raw per-paper reported counts)
    β€’ Canine mammary tumor ddPCR (tissue) shows H1047R mutant fraction range 1.08–80.07% (median 21.87%).
    β€’ In a breast cancer cohort, PIK3CA hotspot mutations cluster in exon 9 and exon 20; total counts were 23 mutations in exon 9 and 23 in exon 20, with H1047R accounting for 22/23 of exon 20 mutations.



     Long Explanation


    Visualize Data: Plot H1047R ddPCR mutant fraction range and exon distribution
    What’s plotted (no assumptions): (1) the reported mutant fraction range for ddPCR-detected PIK3CA(H1047R) in FFPE canine mammary tumor tissue, and (2) the exon distribution of PIK3CA hotspot mutations from an invasive breast carcinoma cohort, with H1047R specifically mapped into exon 20.
    1) H1047R ddPCR mutant fraction (FFPE tissue)
    Readout: ddPCR positivity was 26/80 (32.5%) and the reported mutant fraction (A/(A+B)) among positives spans 1.08–80.07% with median 21.87%.
    2) Exon distribution of PIK3CA hotspots (context for where H1047R sits)
    The breast carcinoma cohort reported 23 hotspot mutations in exon 9 and 23 hotspot mutations in exon 20.
    2b) Within exon 20: where H1047R dominates
    The same cohort reports H1047R as 22 total cases, with only 1 additional exon 20 hotspot case (H1047L), implying H1047R accounts for most exon 20 hotspot mutations in that dataset.
    Skeptical notes / blind spots (what these plots cannot prove)
    • Cross-study biology mixing: the ddPCR mutant fraction plot is from a canine mammary tumor study, while exon distribution is from a human breast cancer cohort; they are plotted together for anatomical/exon context only, not to infer concordance across species.
    • No per-sample distribution: the ddPCR figure uses the reported min–median–max, not individual ddPCR mutant fraction values, so it cannot reveal modality, clustering, or measurement heteroscedasticity within positives.
    • Exon distribution depends on cohort design: exon counts come from a specific single-institution series and can shift with sampling frame and detection method (e.g., screening strategy and confirmation).


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    Updated: March 28, 2026

     Top Data Sources ExportMCP


     Analysis Wizard



    It extracts the reported H1047R ddPCR min/median/max mutant fractions and exon hotspot counts from the provided papers, then generates Plotly figures showing range summaries and exon-20 breakdown consistent with the reported mutation tallies.



     Hypothesis Graveyard


    The ddPCR mutant fraction range (1.08–80.07%) directly maps to clonal fraction/allele dosage in vivo; rejected because FFPE/cfDNA/assay thresholds can compress or inflate measured fractions relative to true in vivo allele burden (data here are summary statistics only).


    Exon distribution is universal across species; rejected because the exon distribution plot is human cohort data whereas the ddPCR mutant fraction plot is canine tissue ddPCR data.

     Science Art


    Visualize Data: Plot H1047R ddPCR mutant fraction range and exon distribution Science Art

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