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     Long Response


    RfxCas13d multiplex array potency vs single crRNA (TCID50)

    Graphs use only the explicit TCID50 log10 reductions reported in the provided research extraction from: .
    What’s being plotted (strictly from provided data)
    • Y-axis: reported TCID50 reduction as log10 difference versus a non-targeting (NT) control, or versus the experiment’s baseline as described in the extraction.
    • X-axis: crRNA configuration (single vs multiplex array) for RfxCas13d.
    • Limitation: only explicitly provided log-reduction values can be graphed; where the extraction says “≈” or “not specified,” those details are not converted into additional numbers.
    Readout: For A/Turkey/Indiana/22-003707-003 at 24h, the multiplex array (PB1_M4+NP_M10) shows the largest TCID50 log10 reduction (4.39) versus either single crRNA alone (3.2 and 3.3). (All values are taken directly from the provided extraction.)
    Readout (with epistemic caution): This bar chart only includes explicit numeric values present in the extraction. For entries like “no detectable viral load at 24h”, the extraction does not provide a numeric log10 reduction, so that point is not plotted numerically.
    Scientific skepticism: without the underlying TCID50 titers and assay detection limits, we cannot convert “no detectable viral load” into an exact log10 reduction.

    Extracted TCID50 log10 reductions used for plots

    Only values explicitly present in the provided extraction are listed.
    Strain / isolate context (as extracted) Time crRNA configuration TCID50 reduction (log10) Note
    A/Turkey/Indiana/22-003707-003 24h PB1_M4 (single) 3.2 Directly extracted
    A/Turkey/Indiana/22-003707-003 24h NP_M10 (single) 3.3 Directly extracted
    A/Turkey/Indiana/22-003707-003 24h PB1_M4 + NP_M10 (multiplex) 4.39 Directly extracted
    General 24h context (as extracted) 24h PB1_M4 ≈1.4 “≈” kept as approximate indicator
    General 24h context (as extracted) 24h NP_M7 ≈2.3 “≈” kept as approximate indicator
    H5N1 (as extracted) 24h NP_M10 ≈1.4 Directly extracted as approximate
    H5N1 (as extracted) 24h PB1_M4 ≈1.3 Directly extracted as approximate
    H7N7 (as extracted) 24h NP_M10 Not given (no detectable viral load) No numeric log reduction in extraction

    Skeptical interpretation (what could change the conclusion)

    • In vitro only: these TCID50 reductions are measured in cell culture models, so in vivo antiviral efficacy and host toxicity remain unproven by these graphs alone.
    • Detection limits: “no detectable viral load” cannot be converted to an exact log reduction without assay LOD/ULO and actual titers; the plot avoids inventing numbers.
    • Multiplex synergy vs additivity: for the specific isolate shown, multiplex is larger than either single, but the extraction does not provide full dose-response curves, uncertainty (e.g., SD/CI), or replicate-level distributions for every point—so we cannot statistically classify synergy from these visuals alone.
    • Generalization: potency may be strain- and host-context dependent; the extraction flags substrate/cell-type variability and the need to test broader applicability.


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    Updated: April 25, 2026

     Analysis Wizard



    Computes multiplex-minus-single log10 potency differences from extracted TCID50 reductions, organizes by strain/time, and outputs a ranked summary table and effect-size scatter for quick visual comparison.



     Science Art


    Create Graphs: RfxCas13d multiplex array potency vs single crRNA (TCID50) Science Art

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