Biochemistry grounded in raw experimental data

Replot Ent5 FRAP and stopped-flow kinetics — interactive workspace

I can replot your Ent5 FRAP and stopped-flow kinetics here — please upload the raw data files (CSV, TSV, Excel) or paste the time/intensity arrays. Until you provide raw traces, the panels below are interactive placeholders that will automatically accept and plot uploaded data. If you want me to run a full automated re-analysis (curve fits, parameter extraction, bootstrapped confidence intervals), click the Run AI Scientist Analysis button.

FRAP traces (Ent5) — placeholder

Stopped-flow kinetics (Ent5) — placeholder

Next steps & recommended analyses

• Upload raw time-series CSV/TSV/Excel for each FRAP and stopped-flow run (columns: Time, Signal, optionally Condition/Replicate).
• After upload, I can: normalize FRAP traces (pre-bleach baseline, bleach-point correction), fit recovery to single/multi-exponential models, extract mobile fraction and t1/2 (half-time), and produce bootstrap CIs.
• For stopped-flow, I can fit single/multi-exponential rises/decays, extract observed rate constants (kobs), and plot kobs vs ligand concentration to estimate kon/koff or Kd when appropriate.

If you prefer, paste here (or upload) two example CSVs named like Ent5_FRAP.csv and Ent5_stoppedflow.csv with first two columns Time and Signal and I will replot, fit, and return publication-quality figures and fitted parameter tables.