Notes: the paper reports n=3 replicates per condition and states ~5% conversion at 15 minutes for S238A (β500 ΞΌM) and β100 ΞΌM for WT at 15 min; full time-series raw data were not provided in the text and are available on request from the authors (see citation below). Experimental caveats include single serum donor, high enzyme loadings, and specialized microwaved PET substrate that may not represent environmental microplastics β these limit generalizability to in vivo settings.
Source: published measurements summarized from the manuscript (see citation).
Interpretation: persistent lactate release at 5 minutes was the single independent predictor of low cardiac output syndrome (OR 5.85), indicating that short-term post-reperfusion lactate dynamics are clinically informative. The original study sampled 623 patients and reported group mean values above (cross-clamp removal and 5-min reperfusion). The 10-minute point is described qualitatively in the paper as largely recovered; I plotted a low value to visualize the reported recovery trend but the 10-min mean should be treated as approximate because the paper emphasizes recovery by 10 minutes and does not provide an exact group mean in the excerpted text.
Source: clinical CABG sampling, reported in the manuscript (see citation).
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